8.4.5. Sample redilution

One potential advantage of Sebia FLC ELISA assays are their broad measuring ranges, which reduce the requirement for sample dilution. Jacobs et al.[1178] reported that the proportion of κ and λ samples that required redilution was 33% and 30% for Freelite assays on the BN™II, compared to 9% and 7% for Sebia FLC ELISA assays. However, other studies have reported lower redilution rates for Freelite assays on Binding Site instruments (Section 7.3). When considering the effect of sample redilution on laboratory workflow, assay time should also be taken into account; Freelite immunoassays are far more rapid than Sebia FLC ELISA protocols, with an approximate assay time of 15 minutes vs. 2 hours.