Berlanga et al.  re-analysed the data reported by Jacobs et al., and plotted the percentage increase in sFLC levels relative to controls for both assays (Figure 8.7). Freelite measured a progressive elevation of κ over λ FLC with worsening renal function, which resulted in some patients having an abnormal κ/λ sFLC ratio. By contrast, N Latex FLC assays indicated an approximately equal increase of both FLCs throughout CKD1-4, but a sharp increase in λ FLC levels in the fifth group, that comprised both CKD5 and dialysis patients. One potential explanation for this anomaly was provided by Kennard et al.  who compared pre- and post-hemodialysis sFLC concentrations in 105 patients with end-stage kidney disease and no known lymphoplasmacytic disorder. Pre-dialysis λ sFLC concentrations were much higher as measured by the N Latex FLC assays than Freelite, but post-haemodialysis, a larger reduction in λ sFLCs was indicated by the N Latex FLC assays (Table 8.6). This resulted in similar post-dialysis λ sFLC concentrations as measured by both assays. One possibility proposed by the authors is that a positively interfering substance may have been present in pre-dialysis samples that selectively affected the λ N Latex FLC assay (causing an over-read). If this interfering substance was of low molecular weight and was removed by haemodialysis, this would explain the greater fall in λ FLC concentrations indicated by the N Latex FLC assays. Further investigations are now required to try and identify the putative interfering substance and test this hypothesis.
|N Latex FLC||Freelite|
|κ/λ (IQR)||κ |
|Pre-haemodialysis ||130 ||250||0.56 (0.47-0.63)||160||110||1.44 (1.14-1.74)|
Table 8.6. Comparison of Freelite and N Latex sFLC concentrations pre- and post-haemodialysis. Median (interquartile range, IQR) values are reported .