9.4.2. Kit calibrators and controls

Chapter 9

HLC kit calibrators are prepared from pooled normal human sera, and kit controls from human sera containing high concentrations of polyclonal immunoglobulins. Values are assigned to kit calibrators using the internal reference materials (Figure 9.7). This is achieved by turbidimetry/nephelometry over five calibration curves, with the internal reference material assayed at four different dilutions with values across the curve range.