Freelite immunoassays for the measurement of free light chains (FLCs) are optimised for Binding Site Optilite and SPAPLUS analysers. The assays utilise latex enhancement to allow detection of FLCs at low concentrations. The development of Freelite assays, normal ranges, and implementation and interpretation are covered in Chapters 5, 6 and 7, respectively.
Immunoglobulin heavy + light chain immunoassays (Hevylite; HLC) are also optimised for Binding Site instruments. Whilst IgGκ, IgGλ, IgAκ and IgAλ Hevylite assays are antisera-based assays, IgMκ and IgMλ Hevylite assays are latex-enhanced immunoassays. The development of Hevylite assays, normal ranges, and implementation and interpretation are covered in Chapters 9, 10 and 11, respectively.