5.3.3. Haemagglutination assays

Chapter 5

Haemagglutination assays are far more sensitive than immunoelectrophoresis, and provide better assessment of specificity. Sheep red blood cells were sensitised with individual FLCs and purified IgG, IgA and IgM, and tested against the FLC antisera. The results showed that κ and λ FLC antibodies reacted with the appropriately labelled cells at >1:16,000 dilution and at <1:2 against cells coated with the alternate FLCs or intact immunoglobulins (Figure 5.4).